Chikungunya virus capsid protein contains nuclear import and export signals
Identifieur interne : 000B13 ( Main/Exploration ); précédent : 000B12; suivant : 000B14Chikungunya virus capsid protein contains nuclear import and export signals
Auteurs : Saijo Thomas [Allemagne, Australie] ; Jagdish Rai [Inde] ; Lijo John [Allemagne, États-Unis] ; Stephan Schaefer [Allemagne] ; Brigitte M. Pützer [Allemagne] ; Ottmar Herchenröder [Allemagne]Source :
- Virology Journal [ 1743-422X ] ; 2013.
Abstract
Chikungunya virus (CHIKV) is an
EGFP-fusion proteins of CHIKV CP and mutants thereof were created and used to monitor their intracellular localization. Binding of cellular proteins was confirmed in pull-down assays with purified CP using co-immuoprecipitation. Nuclear localization was demonstrated in a virus-free system using fluorescence microscopy.
Here we show that CHIKV CP is a nuclear-cytoplasmic shuttling protein with an active NLS that binds to karyopherin α (Karα) for its nuclear translocation. We also found that the Karα4 C-terminal NLS binding site is sufficient for this interaction. We further demonstrate that CHIKV CP interacts directly with the export receptor CRM1 to transport this viral protein out of the nucleus via a nuclear export signal (NES). The CHIKV CP NES was mapped between amino acids 143 and 155 of CP. Deduced from
We were able to show that in a virus-free system that the CHIKV capsid protein contains both, a NLS and a NES, and that it is actively transported between the cytoplasma and the nucleus. We conclude that CHIKV CP has the ability to shuttle via interaction with karyopherins for its nuclear import and,
Url:
DOI: 10.1186/1743-422X-10-269
PubMed: 23984714
PubMed Central: 3765696
Affiliations:
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Pützer</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock</wicri:regionArea><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>Rostock</wicri:noRegion></affiliation></author><author><name sortKey="Herchenroder, Ottmar" sort="Herchenroder, Ottmar" uniqKey="Herchenroder O" first="Ottmar" last="Herchenröder">Ottmar Herchenröder</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock</wicri:regionArea><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>Rostock</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23984714</idno><idno type="pmc">3765696</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765696</idno><idno type="RBID">PMC:3765696</idno><idno type="doi">10.1186/1743-422X-10-269</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000407</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000407</idno><idno type="wicri:Area/Pmc/Curation">000407</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000407</idno><idno type="wicri:Area/Pmc/Checkpoint">000474</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000474</idno><idno type="wicri:Area/Ncbi/Merge">000346</idno><idno type="wicri:Area/Ncbi/Curation">000346</idno><idno type="wicri:Area/Ncbi/Checkpoint">000346</idno><idno type="wicri:Area/Main/Merge">000B15</idno><idno type="wicri:Area/Main/Curation">000B13</idno><idno type="wicri:Area/Main/Exploration">000B13</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Chikungunya virus capsid protein contains nuclear import and export signals</title><author><name sortKey="Thomas, Saijo" sort="Thomas, Saijo" uniqKey="Thomas S" first="Saijo" last="Thomas">Saijo Thomas</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock</wicri:regionArea><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>Rostock</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="I3">Current address: School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Current address: School of Biological Sciences, Monash University, Clayton, VIC 3800</wicri:regionArea><wicri:noRegion>VIC 3800</wicri:noRegion></affiliation></author><author><name sortKey="Rai, Jagdish" sort="Rai, Jagdish" uniqKey="Rai J" first="Jagdish" last="Rai">Jagdish Rai</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Molecular Biology and Bioinformatics, Tripura University Suryamaninagar, Tripura 799022, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Molecular Biology and Bioinformatics, Tripura University Suryamaninagar, Tripura 799022</wicri:regionArea><wicri:noRegion>Tripura 799022</wicri:noRegion></affiliation></author><author><name sortKey="John, Lijo" sort="John, Lijo" uniqKey="John L" first="Lijo" last="John">Lijo John</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock</wicri:regionArea><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>Rostock</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="I4">Current address: MCDB, University of California, Santa Barbara, CA 93106-9625, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Current address: MCDB, University of California, Santa Barbara, CA 93106-9625</wicri:regionArea><wicri:noRegion>CA 93106-9625</wicri:noRegion></affiliation></author><author><name sortKey="Schaefer, Stephan" sort="Schaefer, Stephan" uniqKey="Schaefer S" first="Stephan" last="Schaefer">Stephan Schaefer</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock</wicri:regionArea><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>Rostock</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="I5">Current address: LADR GmbH Medizinisches Versorgungszentrum Mecklenburg-Vorpommern, Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Current address: LADR GmbH Medizinisches Versorgungszentrum Mecklenburg-Vorpommern, Rostock</wicri:regionArea><wicri:noRegion>Rostock</wicri:noRegion><wicri:noRegion>Rostock</wicri:noRegion><wicri:noRegion>Rostock</wicri:noRegion></affiliation></author><author><name sortKey="Putzer, Brigitte M" sort="Putzer, Brigitte M" uniqKey="Putzer B" first="Brigitte M" last="Pützer">Brigitte M. Pützer</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock</wicri:regionArea><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>Rostock</wicri:noRegion></affiliation></author><author><name sortKey="Herchenroder, Ottmar" sort="Herchenroder, Ottmar" uniqKey="Herchenroder O" first="Ottmar" last="Herchenröder">Ottmar Herchenröder</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock</wicri:regionArea><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>18057, Rostock</wicri:noRegion><wicri:noRegion>Rostock</wicri:noRegion></affiliation></author></analytic><series><title level="j">Virology Journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Chikungunya virus (CHIKV) is an <italic>alphavirus</italic> of the <italic>Togaviridae</italic> family. After autoproteolytic cleavage, the CHIKV capsid protein (CP) is involved in RNA binding and assembly of the viral particle. The monomeric CP is approximately 30 kDa in size and is small enough for passive transport through nuclear pores. Some <italic>alphavirus</italic>es are found to harbor nuclear localization signals (NLS) and transport of these proteins between cellular compartments was shown to be energy dependent. The active nuclear import of cytoplasmic proteins is mediated by karyopherins and their export by exportins. As nuclear and cytoplasmic trafficking may play a role in the life cycle of CHIKV, we have sought to identify nuclear localization and nuclear export signals in CHIKV CP in a virus-free system.</p></sec><sec><title>Methods</title><p>EGFP-fusion proteins of CHIKV CP and mutants thereof were created and used to monitor their intracellular localization. Binding of cellular proteins was confirmed in pull-down assays with purified CP using co-immuoprecipitation. Nuclear localization was demonstrated in a virus-free system using fluorescence microscopy.</p></sec><sec><title>Results</title><p>Here we show that CHIKV CP is a nuclear-cytoplasmic shuttling protein with an active NLS that binds to karyopherin α (Karα) for its nuclear translocation. We also found that the Karα4 C-terminal NLS binding site is sufficient for this interaction. We further demonstrate that CHIKV CP interacts directly with the export receptor CRM1 to transport this viral protein out of the nucleus via a nuclear export signal (NES). The CHIKV CP NES was mapped between amino acids 143 and 155 of CP. Deduced from <italic>in silico</italic> analyses we found that the NES has a mode of binding similar to the snurportin-1 CRM1 complex.</p></sec><sec><title>Conclusions</title><p>We were able to show that in a virus-free system that the CHIKV capsid protein contains both, a NLS and a NES, and that it is actively transported between the cytoplasma and the nucleus. We conclude that CHIKV CP has the ability to shuttle via interaction with karyopherins for its nuclear import and, <italic>vice versa</italic>, by CRM1-dependent nuclear export.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Powers, Am" uniqKey="Powers A">AM Powers</name></author><author><name sortKey="Logue, Ch" uniqKey="Logue C">CH Logue</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schwartz, O" uniqKey="Schwartz O">O Schwartz</name></author><author><name sortKey="Albert, Ml" uniqKey="Albert M">ML Albert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thomas, S" uniqKey="Thomas S">S Thomas</name></author><author><name sortKey="Rai, J" uniqKey="Rai J">J Rai</name></author><author><name sortKey="John, L" uniqKey="John L">L John</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Günther</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Putzer, Bm" uniqKey="Putzer B">BM Pützer</name></author><author><name sortKey="Schaefer, S" uniqKey="Schaefer S">S Schaefer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Favre, D" uniqKey="Favre D">D Favre</name></author><author><name sortKey="Studer, E" uniqKey="Studer E">E Studer</name></author><author><name sortKey="Michel, Mr" uniqKey="Michel M">MR Michel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Atasheva, S" uniqKey="Atasheva S">S Atasheva</name></author><author><name sortKey="Fish, A" uniqKey="Fish A">A Fish</name></author><author><name sortKey="Fornerod, M" uniqKey="Fornerod M">M Fornerod</name></author><author><name sortKey="Frolova, Ei" uniqKey="Frolova E">EI Frolova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vasu, Sk" uniqKey="Vasu S">SK Vasu</name></author><author><name sortKey="Forbes, Dj" uniqKey="Forbes D">DJ Forbes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paine, Pl" uniqKey="Paine P">PL Paine</name></author><author><name sortKey="Moore, Lc" uniqKey="Moore L">LC Moore</name></author><author><name sortKey="Horowitz, Sb" uniqKey="Horowitz S">SB Horowitz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lange, A" uniqKey="Lange A">A Lange</name></author><author><name sortKey="Mills, Re" uniqKey="Mills R">RE Mills</name></author><author><name sortKey="Lange, Cj" uniqKey="Lange C">CJ Lange</name></author><author><name sortKey="Stewart, M" uniqKey="Stewart M">M Stewart</name></author><author><name sortKey="Devine, Se" uniqKey="Devine S">SE Devine</name></author><author><name sortKey="Corbett, Ah" uniqKey="Corbett A">AH Corbett</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dingwall, C" uniqKey="Dingwall C">C Dingwall</name></author><author><name sortKey="Laskey, Ra" uniqKey="Laskey R">RA Laskey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dingwall, C" uniqKey="Dingwall C">C Dingwall</name></author><author><name sortKey="Sharnick, Sv" uniqKey="Sharnick S">SV Sharnick</name></author><author><name sortKey="Laskey, Ra" uniqKey="Laskey R">RA Laskey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalderon, D" uniqKey="Kalderon D">D Kalderon</name></author><author><name sortKey="Roberts, Bl" uniqKey="Roberts B">BL Roberts</name></author><author><name sortKey="Richardson, Wd" uniqKey="Richardson W">WD Richardson</name></author><author><name sortKey="Smith, Ae" uniqKey="Smith A">AE Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lanford, Re" uniqKey="Lanford R">RE Lanford</name></author><author><name sortKey="Butel, Js" uniqKey="Butel J">JS Butel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Turner, Jg" uniqKey="Turner J">JG Turner</name></author><author><name sortKey="Sullivan, Dm" uniqKey="Sullivan D">DM Sullivan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nigg, Ea" uniqKey="Nigg E">EA Nigg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Michel, Mr" uniqKey="Michel M">MR Michel</name></author><author><name sortKey="Elgizoli, M" uniqKey="Elgizoli M">M Elgizoli</name></author><author><name sortKey="Dai, Y" uniqKey="Dai Y">Y Dai</name></author><author><name sortKey="Jakob, R" uniqKey="Jakob R">R Jakob</name></author><author><name sortKey="Koblet, H" uniqKey="Koblet H">H Koblet</name></author><author><name sortKey="Arrigo, Ap" uniqKey="Arrigo A">AP Arrigo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melen, K" uniqKey="Melen K">K Melen</name></author><author><name sortKey="Fagerlund, R" uniqKey="Fagerlund R">R Fagerlund</name></author><author><name sortKey="Franke, J" uniqKey="Franke J">J Franke</name></author><author><name sortKey="Kohler, M" uniqKey="Kohler M">M Köhler</name></author><author><name sortKey="Kinnunen, L" uniqKey="Kinnunen L">L Kinnunen</name></author><author><name sortKey="Julkunen, I" uniqKey="Julkunen I">I Julkunen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Conti, E" uniqKey="Conti E">E Conti</name></author><author><name sortKey="Uy, M" uniqKey="Uy M">M Uy</name></author><author><name sortKey="Leighton, L" uniqKey="Leighton L">L Leighton</name></author><author><name sortKey="Blobel, G" uniqKey="Blobel G">G Blobel</name></author><author><name sortKey="Kuriyan, J" uniqKey="Kuriyan J">J Kuriyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fontes, Mr" uniqKey="Fontes M">MR Fontes</name></author><author><name sortKey="Teh, T" uniqKey="Teh T">T Teh</name></author><author><name sortKey="Kobe, B" uniqKey="Kobe B">B Kobe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fagerlund, R" uniqKey="Fagerlund R">R Fagerlund</name></author><author><name sortKey="Kinnunen, L" uniqKey="Kinnunen L">L Kinnunen</name></author><author><name sortKey="Kohler, M" uniqKey="Kohler M">M Köhler</name></author><author><name sortKey="Julkunen, I" uniqKey="Julkunen I">I Julkunen</name></author><author><name sortKey="Melen, K" uniqKey="Melen K">K Melen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kudo, N" uniqKey="Kudo N">N Kudo</name></author><author><name sortKey="Wolff, B" uniqKey="Wolff B">B Wolff</name></author><author><name sortKey="Sekimoto, T" uniqKey="Sekimoto T">T Sekimoto</name></author><author><name sortKey="Schreiner, Ep" uniqKey="Schreiner E">EP Schreiner</name></author><author><name sortKey="Yoneda, Y" uniqKey="Yoneda Y">Y Yoneda</name></author><author><name sortKey="Yanagida, M" uniqKey="Yanagida M">M Yanagida</name></author><author><name sortKey="Horinouchi, S" uniqKey="Horinouchi S">S Horinouchi</name></author><author><name sortKey="Yoshida, M" uniqKey="Yoshida M">M Yoshida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garmashova, N" uniqKey="Garmashova N">N Garmashova</name></author><author><name sortKey="Gorchakov, R" uniqKey="Gorchakov R">R Gorchakov</name></author><author><name sortKey="Volkova, E" uniqKey="Volkova E">E Volkova</name></author><author><name sortKey="Paessler, S" uniqKey="Paessler S">S Paessler</name></author><author><name sortKey="Frolova, E" uniqKey="Frolova E">E Frolova</name></author><author><name sortKey="Frolov, I" uniqKey="Frolov I">I Frolov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bourai, M" uniqKey="Bourai M">M Bourai</name></author><author><name sortKey="Lucas Hourani, M" uniqKey="Lucas Hourani M">M Lucas-Hourani</name></author><author><name sortKey="Gad, Hh" uniqKey="Gad H">HH Gad</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Jacob, Y" uniqKey="Jacob Y">Y Jacob</name></author><author><name sortKey="Tafforeau, L" uniqKey="Tafforeau L">L Tafforeau</name></author><author><name sortKey="Cassonnet, P" uniqKey="Cassonnet P">P Cassonnet</name></author><author><name sortKey="Jones, Lm" uniqKey="Jones L">LM Jones</name></author><author><name sortKey="Judith, D" uniqKey="Judith D">D Judith</name></author><author><name sortKey="Couderc, T" uniqKey="Couderc T">T Couderc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kohler, M" uniqKey="Kohler M">M Köhler</name></author><author><name sortKey="Gorlich, D" uniqKey="Gorlich D">D Görlich</name></author><author><name sortKey="Hartmann, E" uniqKey="Hartmann E">E Hartmann</name></author><author><name sortKey="Franke, J" uniqKey="Franke J">J Franke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kohler, M" uniqKey="Kohler M">M Köhler</name></author><author><name sortKey="Speck, C" uniqKey="Speck C">C Speck</name></author><author><name sortKey="Christiansen, M" uniqKey="Christiansen M">M Christiansen</name></author><author><name sortKey="Bischoff, Fr" uniqKey="Bischoff F">FR Bischoff</name></author><author><name sortKey="Prehn, S" uniqKey="Prehn S">S Prehn</name></author><author><name sortKey="Haller, H" uniqKey="Haller H">H Haller</name></author><author><name sortKey="Gorlich, D" uniqKey="Gorlich D">D Görlich</name></author><author><name sortKey="Hartmann, E" uniqKey="Hartmann E">E Hartmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miyamoto, Y" uniqKey="Miyamoto Y">Y Miyamoto</name></author><author><name sortKey="Imamoto, N" uniqKey="Imamoto N">N Imamoto</name></author><author><name sortKey="Sekimoto, T" uniqKey="Sekimoto T">T Sekimoto</name></author><author><name sortKey="Tachibana, T" uniqKey="Tachibana T">T Tachibana</name></author><author><name sortKey="Seki, T" uniqKey="Seki T">T Seki</name></author><author><name sortKey="Tada, S" uniqKey="Tada S">S Tada</name></author><author><name sortKey="Enomoto, T" uniqKey="Enomoto T">T Enomoto</name></author><author><name sortKey="Yoneda, Y" uniqKey="Yoneda Y">Y Yoneda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Welch, K" uniqKey="Welch K">K Welch</name></author><author><name sortKey="Franke, J" uniqKey="Franke J">J Franke</name></author><author><name sortKey="Kohler, M" uniqKey="Kohler M">M Köhler</name></author><author><name sortKey="Macara, Ig" uniqKey="Macara I">IG Macara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guttler, T" uniqKey="Guttler T">T Güttler</name></author><author><name sortKey="Madl, T" uniqKey="Madl T">T Madl</name></author><author><name sortKey="Neumann, P" uniqKey="Neumann P">P Neumann</name></author><author><name sortKey="Deichsel, D" uniqKey="Deichsel D">D Deichsel</name></author><author><name sortKey="Corsini, L" uniqKey="Corsini L">L Corsini</name></author><author><name sortKey="Monecke, T" uniqKey="Monecke T">T Monecke</name></author><author><name sortKey="Ficner, R" uniqKey="Ficner R">R Ficner</name></author><author><name sortKey="Sattler, M" uniqKey="Sattler M">M Sattler</name></author><author><name sortKey="Gorlich, D" uniqKey="Gorlich D">D Görlich</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hantschel, O" uniqKey="Hantschel O">O Hantschel</name></author><author><name sortKey="Wiesner, S" uniqKey="Wiesner S">S Wiesner</name></author><author><name sortKey="Guttler, T" uniqKey="Guttler T">T Güttler</name></author><author><name sortKey="Mackereth, Cd" uniqKey="Mackereth C">CD Mackereth</name></author><author><name sortKey="Rix, Ll" uniqKey="Rix L">LL Rix</name></author><author><name sortKey="Mikes, Z" uniqKey="Mikes Z">Z Mikes</name></author><author><name sortKey="Dehne, J" uniqKey="Dehne J">J Dehne</name></author><author><name sortKey="Gorlich, D" uniqKey="Gorlich D">D Görlich</name></author><author><name sortKey="Sattler, M" uniqKey="Sattler M">M Sattler</name></author><author><name sortKey="Superti Furga, G" uniqKey="Superti Furga G">G Superti-Furga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taagepera, S" uniqKey="Taagepera S">S Taagepera</name></author><author><name sortKey="Mcdonald, D" uniqKey="Mcdonald D">D McDonald</name></author><author><name sortKey="Loeb, Je" uniqKey="Loeb J">JE Loeb</name></author><author><name sortKey="Whitaker, Ll" uniqKey="Whitaker L">LL Whitaker</name></author><author><name sortKey="Mcelroy, Ak" uniqKey="Mcelroy A">AK McElroy</name></author><author><name sortKey="Wang, Jy" uniqKey="Wang J">JY Wang</name></author><author><name sortKey="Hope, Tj" uniqKey="Hope T">TJ Hope</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mattaj, Iw" uniqKey="Mattaj I">IW Mattaj</name></author><author><name sortKey="Muller, Cw" uniqKey="Muller C">CW Müller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kutay, U" uniqKey="Kutay U">U Kutay</name></author><author><name sortKey="Guttinger, S" uniqKey="Guttinger S">S Güttinger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Engelsma, D" uniqKey="Engelsma D">D Engelsma</name></author><author><name sortKey="Valle, N" uniqKey="Valle N">N Valle</name></author><author><name sortKey="Fish, A" uniqKey="Fish A">A Fish</name></author><author><name sortKey="Salome, N" uniqKey="Salome N">N Salome</name></author><author><name sortKey="Almendral, Jm" uniqKey="Almendral J">JM Almendral</name></author><author><name sortKey="Fornerod, M" uniqKey="Fornerod M">M Fornerod</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corredor, Ag" uniqKey="Corredor A">AG Corredor</name></author><author><name sortKey="St Louis, Mc" uniqKey="St Louis M">MC St-Louis</name></author><author><name sortKey="Archambault, D" uniqKey="Archambault D">D Archambault</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Indik, S" uniqKey="Indik S">S Indik</name></author><author><name sortKey="Gunzburg, Wh" uniqKey="Gunzburg W">WH Gunzburg</name></author><author><name sortKey="Salmons, B" uniqKey="Salmons B">B Salmons</name></author><author><name sortKey="Rouault, F" uniqKey="Rouault F">F Rouault</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harris, Me" uniqKey="Harris M">ME Harris</name></author><author><name sortKey="Gontarek, Rr" uniqKey="Gontarek R">RR Gontarek</name></author><author><name sortKey="Derse, D" uniqKey="Derse D">D Derse</name></author><author><name sortKey="Hope, Tj" uniqKey="Hope T">TJ Hope</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sharma, K" uniqKey="Sharma K">K Sharma</name></author><author><name sortKey="Akerstrom, S" uniqKey="Akerstrom S">S Akerstrom</name></author><author><name sortKey="Sharma, Ak" uniqKey="Sharma A">AK Sharma</name></author><author><name sortKey="Chow, Vt" uniqKey="Chow V">VT Chow</name></author><author><name sortKey="Teow, S" uniqKey="Teow S">S Teow</name></author><author><name sortKey="Abrenica, B" uniqKey="Abrenica B">B Abrenica</name></author><author><name sortKey="Booth, Sa" uniqKey="Booth S">SA Booth</name></author><author><name sortKey="Booth, Tf" uniqKey="Booth T">TF Booth</name></author><author><name sortKey="Mirazimi, A" uniqKey="Mirazimi A">A Mirazimi</name></author><author><name sortKey="Lal, Sk" uniqKey="Lal S">SK Lal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scheifele, Lz" uniqKey="Scheifele L">LZ Scheifele</name></author><author><name sortKey="Ryan, Ep" uniqKey="Ryan E">EP Ryan</name></author><author><name sortKey="Parent, Lj" uniqKey="Parent L">LJ Parent</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cerutti, A" uniqKey="Cerutti A">A Cerutti</name></author><author><name sortKey="Maillard, P" uniqKey="Maillard P">P Maillard</name></author><author><name sortKey="Minisini, R" uniqKey="Minisini R">R Minisini</name></author><author><name sortKey="Vidalain, Po" uniqKey="Vidalain P">PO Vidalain</name></author><author><name sortKey="Roohvand, F" uniqKey="Roohvand F">F Roohvand</name></author><author><name sortKey="Pecheur, Ei" uniqKey="Pecheur E">EI Pecheur</name></author><author><name sortKey="Pirisi, M" uniqKey="Pirisi M">M Pirisi</name></author><author><name sortKey="Budkowska, A" uniqKey="Budkowska A">A Budkowska</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Connor, Mk" uniqKey="Connor M">MK Connor</name></author><author><name sortKey="Kotchetkov, R" uniqKey="Kotchetkov R">R Kotchetkov</name></author><author><name sortKey="Cariou, S" uniqKey="Cariou S">S Cariou</name></author><author><name sortKey="Resch, A" uniqKey="Resch A">A Resch</name></author><author><name sortKey="Lupetti, R" uniqKey="Lupetti R">R Lupetti</name></author><author><name sortKey="Beniston, Rg" uniqKey="Beniston R">RG Beniston</name></author><author><name sortKey="Melchior, F" uniqKey="Melchior F">F Melchior</name></author><author><name sortKey="Hengst, L" uniqKey="Hengst L">L Hengst</name></author><author><name sortKey="Slingerland, Jm" uniqKey="Slingerland J">JM Slingerland</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ossovskaya, V" uniqKey="Ossovskaya V">V Ossovskaya</name></author><author><name sortKey="Lim, St" uniqKey="Lim S">ST Lim</name></author><author><name sortKey="Ota, N" uniqKey="Ota N">N Ota</name></author><author><name sortKey="Schlaepfer, Dd" uniqKey="Schlaepfer D">DD Schlaepfer</name></author><author><name sortKey="Ilic, D" uniqKey="Ilic D">D Ilic</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eulalio, A" uniqKey="Eulalio A">A Eulalio</name></author><author><name sortKey="Nunes Correia, I" uniqKey="Nunes Correia I">I Nunes-Correia</name></author><author><name sortKey="Carvalho, Al" uniqKey="Carvalho A">AL Carvalho</name></author><author><name sortKey="Faro, C" uniqKey="Faro C">C Faro</name></author><author><name sortKey="Citovsky, V" uniqKey="Citovsky V">V Citovsky</name></author><author><name sortKey="Salas, J" uniqKey="Salas J">J Salas</name></author><author><name sortKey="Salas, Ml" uniqKey="Salas M">ML Salas</name></author><author><name sortKey="Simoes, S" uniqKey="Simoes S">S Simoes</name></author><author><name sortKey="De Lima, Mc" uniqKey="De Lima M">MC de Lima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Akhrymuk, I" uniqKey="Akhrymuk I">I Akhrymuk</name></author><author><name sortKey="Kulemzin, Sv" uniqKey="Kulemzin S">SV Kulemzin</name></author><author><name sortKey="Frolova, Ei" uniqKey="Frolova E">EI Frolova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Agapov, Ev" uniqKey="Agapov E">EV Agapov</name></author><author><name sortKey="Frolov, I" uniqKey="Frolov I">I Frolov</name></author><author><name sortKey="Lindenbach, Bd" uniqKey="Lindenbach B">BD Lindenbach</name></author><author><name sortKey="Pragai, Bm" uniqKey="Pragai B">BM Pragai</name></author><author><name sortKey="Schlesinger, S" uniqKey="Schlesinger S">S Schlesinger</name></author><author><name sortKey="Rice, Cm" uniqKey="Rice C">CM Rice</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frolova, Ei" uniqKey="Frolova E">EI Frolova</name></author><author><name sortKey="Fayzulin, Rz" uniqKey="Fayzulin R">RZ Fayzulin</name></author><author><name sortKey="Cook, Sh" uniqKey="Cook S">SH Cook</name></author><author><name sortKey="Griffin, De" uniqKey="Griffin D">DE Griffin</name></author><author><name sortKey="Rice, Cm" uniqKey="Rice C">CM Rice</name></author><author><name sortKey="Frolov, I" uniqKey="Frolov I">I Frolov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Atasheva, S" uniqKey="Atasheva S">S Atasheva</name></author><author><name sortKey="Garmashova, N" uniqKey="Garmashova N">N Garmashova</name></author><author><name sortKey="Frolov, I" uniqKey="Frolov I">I Frolov</name></author><author><name sortKey="Frolova, E" uniqKey="Frolova E">E Frolova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Follett, Ea" uniqKey="Follett E">EA Follett</name></author><author><name sortKey="Pringle, Cr" uniqKey="Pringle C">CR Pringle</name></author><author><name sortKey="Pennington, Th" uniqKey="Pennington T">TH Pennington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Erwin, C" uniqKey="Erwin C">C Erwin</name></author><author><name sortKey="Brown, Dt" uniqKey="Brown D">DT Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kowalzik, S" uniqKey="Kowalzik S">S Kowalzik</name></author><author><name sortKey="Xuan, Nv" uniqKey="Xuan N">NV Xuan</name></author><author><name sortKey="Weissbrich, B" uniqKey="Weissbrich B">B Weissbrich</name></author><author><name sortKey="Scheiner, B" uniqKey="Scheiner B">B Scheiner</name></author><author><name sortKey="Schied, T" uniqKey="Schied T">T Schied</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Muller, A" uniqKey="Muller A">A Müller</name></author><author><name sortKey="Stich, A" uniqKey="Stich A">A Stich</name></author><author><name sortKey="Rethwilm, A" uniqKey="Rethwilm A">A Rethwilm</name></author><author><name sortKey="Bodem, J" uniqKey="Bodem J">J Bodem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takeda, A" uniqKey="Takeda A">A Takeda</name></author><author><name sortKey="Sarma, Nj" uniqKey="Sarma N">NJ Sarma</name></author><author><name sortKey="Abdul Nabi, Am" uniqKey="Abdul Nabi A">AM Abdul-Nabi</name></author><author><name sortKey="Yaseen, Nr" uniqKey="Yaseen N">NR Yaseen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shaw, Ml" uniqKey="Shaw M">ML Shaw</name></author><author><name sortKey="Cardenas, Wb" uniqKey="Cardenas W">WB Cardenas</name></author><author><name sortKey="Zamarin, D" uniqKey="Zamarin D">D Zamarin</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author><author><name sortKey="Basler, Cf" uniqKey="Basler C">CF Basler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author><author><name sortKey="O Eill, Re" uniqKey="O Eill R">RE O’Neill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sheng, Z" uniqKey="Sheng Z">Z Sheng</name></author><author><name sortKey="Lewis, Ja" uniqKey="Lewis J">JA Lewis</name></author><author><name sortKey="Chirico, Wj" uniqKey="Chirico W">WJ Chirico</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Offman, Mn" uniqKey="Offman M">MN Offman</name></author><author><name sortKey="Tournier, Al" uniqKey="Tournier A">AL Tournier</name></author><author><name sortKey="Bates, Pa" uniqKey="Bates P">PA Bates</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guex, N" uniqKey="Guex N">N Guex</name></author><author><name sortKey="Peitsch, Mc" uniqKey="Peitsch M">MC Peitsch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="London, N" uniqKey="London N">N London</name></author><author><name sortKey="Raveh, B" uniqKey="Raveh B">B Raveh</name></author><author><name sortKey="Cohen, E" uniqKey="Cohen E">E Cohen</name></author><author><name sortKey="Fathi, G" uniqKey="Fathi G">G Fathi</name></author><author><name sortKey="Schueler Furman, O" uniqKey="Schueler Furman O">O Schueler-Furman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mashiach, E" uniqKey="Mashiach E">E Mashiach</name></author><author><name sortKey="Schneidman Duhovny, D" uniqKey="Schneidman Duhovny D">D Schneidman-Duhovny</name></author><author><name sortKey="Andrusier, N" uniqKey="Andrusier N">N Andrusier</name></author><author><name sortKey="Nussinov, R" uniqKey="Nussinov R">R Nussinov</name></author><author><name sortKey="Wolfson, Hj" uniqKey="Wolfson H">HJ Wolfson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Andrusier, N" uniqKey="Andrusier N">N Andrusier</name></author><author><name sortKey="Nussinov, R" uniqKey="Nussinov R">R Nussinov</name></author><author><name sortKey="Wolfson, Hj" uniqKey="Wolfson H">HJ Wolfson</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>Inde</li><li>États-Unis</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Thomas, Saijo" sort="Thomas, Saijo" uniqKey="Thomas S" first="Saijo" last="Thomas">Saijo Thomas</name></noRegion><name sortKey="Herchenroder, Ottmar" sort="Herchenroder, Ottmar" uniqKey="Herchenroder O" first="Ottmar" last="Herchenröder">Ottmar Herchenröder</name><name sortKey="John, Lijo" sort="John, Lijo" uniqKey="John L" first="Lijo" last="John">Lijo John</name><name sortKey="Putzer, Brigitte M" sort="Putzer, Brigitte M" uniqKey="Putzer B" first="Brigitte M" last="Pützer">Brigitte M. Pützer</name><name sortKey="Schaefer, Stephan" sort="Schaefer, Stephan" uniqKey="Schaefer S" first="Stephan" last="Schaefer">Stephan Schaefer</name><name sortKey="Schaefer, Stephan" sort="Schaefer, Stephan" uniqKey="Schaefer S" first="Stephan" last="Schaefer">Stephan Schaefer</name></country><country name="Australie"><noRegion><name sortKey="Thomas, Saijo" sort="Thomas, Saijo" uniqKey="Thomas S" first="Saijo" last="Thomas">Saijo Thomas</name></noRegion></country><country name="Inde"><noRegion><name sortKey="Rai, Jagdish" sort="Rai, Jagdish" uniqKey="Rai J" first="Jagdish" last="Rai">Jagdish Rai</name></noRegion></country><country name="États-Unis"><noRegion><name sortKey="John, Lijo" sort="John, Lijo" uniqKey="John L" first="Lijo" last="John">Lijo John</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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